Используя данные предыдущих разработок пилотных способов получения фрагмента стрептокиназы СК1-63 предложена новая упрощенная технология производства фрагмента из нативной стрептокиназы Streptococcus pyogenes с удовлетворительным выходом и высокой чистотой целевого фрагмента. Подтверждено, что фрагмент СК1-63 стимулирует освобождение тканевого активатора плазминогена из эндотелиальных клеток как в экспериментах in vitro (культура эндотелиальных клеток HUVEC), так и in vivo (модель гемостаза кролей). Вместе с тем фрагмент практически не имеет иммуногенности и никакого другого негативного воздействия на систему гемостаза кроля в противоположность стрептокиназе. Предполагается, что благодаря быстрому всасыванию через стенку кишечника в кровь фрагмент СК1-63 может претендовать на роль первого per oral антитромботического профилактического лекарственного препарата.

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стрептокиназа, фрагмент СК1-63, влияние на освобождение ТАП, культура эндотелиальных клеток