Capabilities of fill and draw cultivation method for obtaining biomass of the producer strain of granulocyte-macrophage colony-stimulating factor

Abstract

In this experimental study we have assessed the capabilities of cultivation of the recombinant Escherichia coli SG 20050/p280_2GM strain, a producer of granulocyte-macrophage colony-stimulating factor (rhGM-CSF) by fill and draw method for obtaining biomass in a 15 l Biotron fermenter. It has been determined that the replacement of 70% of the culture liquid with an equal amount of new fresh nutrient medium is optimal to achieve the maximum level of protein expression. The cultivation time in subsequent cycles is reduced by 3 – 4 hours. By using sequential fill and draw cultivation of 4 cycles, the time for obtaining biomass is reduced by 1.8 times as compared to 4 cycles of batch cultivation. Under these conditions, the productivity of the strain in terms of the specific gravity (3.5 – 4.0 g/l) and the content of the target protein (20 – 23%) remains extant for no less than 3 cycles.

DOI: 10.30906/2073-8099-2021-13-5-9-13